Premier League players and club staff will need to take a lateral flow test every day in order to get into training grounds as part of new measures agreed on Tuesday.
The action is aimed at stemming the rising number of positive cases.
The Premier League confirmed on Monday there had been a record 42 positives over the previous seven days.
It is the highest figure recorded since Covid testing began as part of Project Restart during the 2019-20 season.
Previously, the most positive tests returned in a week this season was 16 from 16-22 August, when there were 3,060 tests.
Outbreaks at Tottenham and Manchester United have caused the postponement of two league games within the space of three days, while Brighton, Leicester, Aston Villa and Norwich have all confirmed cases.
In addition to the daily tests to get into training grounds, players and staff will now have a PCR test at least twice a week, which was the case last season, before it was changed to lateral flow tests in the summer.
It is anticipated the measures will be implemented quickly in an attempt to stem the spread of the new Omicron variant of coronavirus. Additional mask wearing, limiting of time spent receiving physical treatment and social distancing are also being advised to reduce the risk of further cases.
With the season about to enter the busy festive programme, clubs will want to avoid postponements which could create a fixture backlog and potentially even leave the league open to demands for rebates from broadcast partners, which had to be paid when the 2019-20 season was suspended at the start of the pandemic.
(BBC Sport)
